EFI 2012

Oral Presentations

Best Abstracts Session - Hall 1a/b/c : Saturday, 2nd June, 14:45-16:45
O1 BACTERIA MODULATE THE CD8+ T CELL EPITOPE REPERTOIRE OF HOST CYTOSOL-EXPOSED PROTEINS TO MANIPULATE THE HOST IMMUNE RESPONSE.
Y Louzoun, Y Maman (Ramat Gan)

O2 KEY ROLES FOR POSITION 156 OF HLA CLASS I MOLECULES IN PEPTIDE LOADING AND ANTIGEN PRESENTATION
S Badrinath, T Huyton, R Blasczyk, C Bade-Doeding (Hannover)

O3 TCR GENE EDITING RESULTS IN EFFECTIVE IMMUNOTHERAPY OF LEUKEMIA WITHOUT THE DEVELOPMENT OF GVHD
E Provasi, P Genovese, A Lombardo, Z Magnani, P Liu, A Reik, V Chu, DE Paschon, L Zhang, J Kuball, B Camisa, A Bondanza, G Casorati, M Ponzoni, F Ciceri, C Bordignon, PD Greenberg, MC Holmes, PD Gregory, L Naldini, C Bonini (Milano)

O4 THE MHC AND HLA ALLELIC DIVERSITY OF THE 1000 GENOME SAMPLES
PA Gourraud, N Cereb, P KhanKhanian, S Yang, R Dunivin, M Feolo, J Rioux, S Hauser, J Oksenberg (San Francisco)

O5 ALLELIC VARIATION IN THE PROTEIN STABILITY OF HLA-DQ SHAPES GENETIC PREDISPOSITION TO AUTOIMMUNITY
H Miyadera, J Ohashi, K Tokunaga (Tokyo)

O6 NON-CYTOLYTIC HY-SPECIFIC CD4+ T CELLS MEDIATE A STRONG GRAFT-VERSUS-LEUKEMIA EFFECT VIA CXCR4 DOWN-REGULATION ON TARGET CELLS
A Bondanza, L Hambach, Z Aghai, M Casucci, B Camisa, K Fleischhauer, B Nijmeijer, F Ciceri, C Bonini, E Goulmy (Milano)

O7 HLA CLASS I HISTAMERS: A NEW TOOL FOR VISUALIZATION AND SELECTION OF FUNCTIONALLY ACTIVE ANTIGEN-SPECIFIC CD8+ T LYMPHOCYTES
S Tischer, K Till, C Figueiredo, O Hiller, B Maecker-Kolhoff, R Geyeregger, S Immenschuh, R Blasczyk, B Eiz-Vesper (Hannover)

O8 CANCER IMMUNOTHERAPY USING IDEAL TUMOR-ASSOCIATED ANTIGENIC PEPTIDES AND HUMAN IPS CELL-DERIVED DENDRITIC CELLS
Y Nishimura, T Nakatsura, Y Nakamura, S Senju (Kumamoto)

O9 TARGETED DISRUPTION OF THE TAP2 GENE IN HUMAN IPS CELLS AND GENERATION OF AN INFINITE DENDRITIC CELL SOURCE THAT IS APPLICABLE TO VACCINATION THERAPY IRRESPECTIVE OF HLA TYPES OF THE PATIENTS
S Senju, M Haruta, Y Tomita, Y Nishimura (Kumamoto)

O10 A BALANCE OF KIR A AND KIR B HAPLOTYPES IN ARCHAIC HUMANS
PJ Norman, L Abi-Rached, P Parham (Stanford)

Oral I -Haematopoietic Stem Cell Transplantation - Hall 1b : Friday, 1st June, 11:00-13:00
O11 HIGH ALLELIC VARIABILITY IN HLA-C MRNA EXPRESSION: PREDOMINANT ASSOCIATION WITH HLA-B-C HAPLOTYPES RATHER THAN WITH -35KB C/T SINGLE NUCLEOTIDE POLYMORPHISM
L Brunet, F Bettens, S Buhler, J Tiercy (Geneva)

O12 IMPACT OF HLA UNIDIRECTIONAL MISMATCHES ON THE OUTCOME OF UNRELATED DONOR HEMATOPOIETIC STEM CELL TRANSPLANTATION
CK Hurley, T Wang, SR Spellman, J Umejiego, M Aljurf, M Askar, M Battiwalla, J Dehn, J Horan, SR Marino, SGE Marsh, D Maurer, M Oudshoorn, J Pidala, W Saber, KR Schultz, V Turner, SJ Lee, A Woolfrey (Minneapolis)

O13 HIGH RESOLUTION HLA TYPING OF BLOOD STEM CELL DONORS WITH NEXT GENERATION SEQUENCING TECHNOLOGY
K Hirv, I Nieberle, S Welzmüller, B Grumbt, T Zacher, H Knabe (Martinsried)

O14 THE NUMBER OF ACCEPTABLE HLA MISMATCHES CAN INCREASE ALMOST THREE FOLD BY MATCHING NOW AVAILABLE CORD BLOOD UNITS FOR NON INHERITED MATERNAL ANTIGENS
JJ van Rood, HG van der Zanden, M Oudshoorn, FH Claas, P Rubinstein (Leiden)

O15 HLA-DRB3 AND HLA-DRB4 MATCHING IS RELEVANT FOR UNRELATED ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION.
M Detrait, V Dubois, M Sobh, S Morisset, F Barraco, F Nicolini, H Labussiere, S Ducastelle, X Thomas, Y Chelghoum, N Tedone, S Rey, M Michallet (LYON)

O16 HLA-DP SPECIFIC CD4+ T CELL RECOGNITION IS AFFECTED BY POLYMORPHISM IN THE ALPHA CHAIN OF THE DP MOLECULE
N Lauterbach, P Crivello, L Wieten, L Zito, M Groeneweg, CE Voorter, K Fleischhauer, MG Tilanus (Maastricht)

O17 SIGNIFICANCE OF KIR RECEPTORS FOR DONOR SELECTION IN HEMATOPOETIC STEM CELL TRANSPLANTATION
R Richter, P Becker, H Serve, G Bug, E Seifried, H Martin, C Seidl (Frankfurt a.M.)

O18 KILLER IMMUNOGLOBULIN-LIKE RECEPTORS AND THEIR HLA CLASS 1 LIGANDS CAN HELP REFINE DONOR SELECTION CRITERIA FOR THALASSEMIA PATIENTS UNDERGOING UNRELATED HEMATOPOIETIC STEM CELL TRANSPLANTATION
R Littera, M Sanna, M Mulargia, R Porcella, P Paganelli, M Serra, R Maddi, V Loi, C Giardini, F Locatelli, G La Nasa, C Carcassi (Cagliari)

O19 ASSESSMENT OF DONOR-DERIVED KILLER IMMUNOGLOBULIN-LIKE RECEPTORS (KIR) BY SEQUENTIAL CDNA GENOTYPING IN NON T CELL DEPLETED HAPLOIDENTICAL STEM CELL-TRANSPLANTED PATIENTS.
A Canossi, A Aureli, T Del Beato, W Arcese, F Papola, S Santarone, R Arriga, P Di Bartolomeo, G Sconocchia (L'Aquila)

O20 INHIBITORY KIR AND NKG2A MODULATE NK CELL ANTI-MYELOMA RESPONSES AT ATMOSPHERIC AND HYPOXIC OXYGEN LEVELS
L Wieten, S Sarkar, CH van Elssen, AC Martens, M van Gelder, KM Rouschop, GM Bos, MG Tilanus (Maastricht)

Oral II - Autoimmunity, Disease Association and Cancer - Hall 1c : Friday, 1st June, 11:00-13:00
O21 IMMUNE SELF-REACTIVITY TRIGGERED BY DRUG-MODIFIED HUMAN LEUKOCYTE ANTIGEN-PEPTIDE PRESENTATION
PT Illing, JP Vivian, NL Dudek, L Kostenko, Z Chen, M Bharadwaj, L Kjer-Nielsen, NP Croft, NA Williamson, AW Purcell, J Rossjohn, J McCluskey (Parkville)

O22 PROTECTIVE HLA MOLECULES DETERMINE INFECTION OUTCOME IN HEPATITIS C VIRUS INFECTION BY PREFERENTIAL PRESENTATION OF PEPTIDES FROM CONSERVED VIRAL PROTEINS
I Hoof, X Rao, D van Baarle, C Kesmir (Utrecht)

O23 TOWARDS THE NEXT GENERATION OF GENETIC STUDIES AND DIAGNOSTICS FOR HLA
PJ Norman, S Norberg, T Royce, M Won, T Dunn, T Mann, N Nemat-Gorgani, LA Guethlein, L Abi-Rached, L Tian, KL Gunderson, M Ronaghi, P Parham (Stanford)

O24 GENETIC RISK SCORE IN MULTIPLE SCLEROSIS: BACK TO THE INDIVIDUAL IN THE POST-GWAS ERA.
N Isobe, V Bakthavachalam, S Cailler, A Santaniello, S Hauser, J Oksenberg, PA Gourraud (San Francisco)

O25 PRONOUNCED KIR MEDIATED INHIBITION IS ASSOCIATED WITH RAPID PROGRESSION OF MALIGNANT MELANOMA.
EJ Naumova, SM Kandilarova, A Paschen, A Sucker, AP Mihaylova, MI Ivanova, D Schadendorf (Sofia)

O26 TUMOR CELL ASSOCIATED HYPOXIA: AN INHIBTORY FACTOR FOR NK CELL ANTI-MYELOMA RESPONSES
S Sarkar, WT Germeraad, EM Steeghs, MG Tilanus, M van Gelder, KM Rouschop, GM Bos, L Wieten (Maastricht)

O27 SELECTIVE IMMUNOGLOBULIN A DEFICIENCY IS ASSOCIATED WITH IFIH1, PVT1, FAS, CDH13 AND KIF3 IN A GENOMEWIDE ASSOCIATION STUDY AND META-ANALYSIS
PG Bronson, AP Manoharan, TR Bhangale, W Ortmann, RC Ferreira, Q Pan-Hammerstrom, L Hammerstrom, RR Graham, TW Behrens (South San Francisco)

O28 IDENTIFICATION OF NOVEL TUMOR-ASSOCIATED ANTIGENIC PEPTIDES RECOGNIZED BY TUMOR-REACTIVE AND HLA-A2- OR A24-RESTRICTED CYTOTOXIC T-LYMPHOCYTES USEFUL FOR IMMUNOTHERAPY OF LUNG CANCER
Y Nishimura, Y Tomita, S Senju, A Irie, T Mori, Y Daigo, T Tsunoda, T Ito, H Nomori, Y Nakamura, H Kohrogi, Y Nishimura (Kumamoto)

O29 IMMUNE ESCAPE STRATEGIES OF A CONTAGIOUS CANCER, DEVIL FACIAL TUMOUR DISEASE
HV Siddle, A Kreiss, C Tovar, K Swift, K Belov, A Pearse, K Skjøadt, GM Woods, J Kaufman (Cambridge)

O30 ACTIVATING KIR AND THE HLA BW4 EPITOPE ARE ASSOCIATED TO DECREASED SUSCEPTIBILITY TO PEMPHIGUS FOLIACEUS, AN AUTOIMMUNE BLISTERING DISEASE OF SKIN
DG Augusto, SC Lobo-Alves, MF Melo, NF Pereira, M Petzl-Erler (Curitiba)

Oral III - Solid Organ Transplantation/Bioinformatics - Hall 1b : Saturday, 2nd June, 11:00-13:00
O31 HLA CLASS I ANTIBODIES ACTIVATE ENDOTHELIAL CELLS AND UP-REGULATE ADHESION MOLECULES; A POTENTIAL LINK BETWEEN ANTIBODIES AND CELLULAR REJECTION
FM Naemi, S Ali, V Carter, JA Kirby (Newcastle Upon Tyne)

O32 SILENCING HLA CLASS I EXPRESSION IN HUMAN CORNEAS TO DECREASE THE RISK OF GRAFT REJECTION AFTER KERATOPLASTY
C Figueiredo, S Vahlsing, M Valting, M Börgel, K Engelmann, R Blasczyk (Hannover)

O33 HIGH RESOLUTION ANALYSIS OF RENAL ALLOGRAFT LOSS - HLA CLASS II MISMATCH AND ANTIBODIES
R Vaughan, P Mobillo, R Collins, J Theron, O Shaw, E Lougee, M Hernadez-Fuentes (London)

O34 THE PREVALENCE AND RELEVANCE OF HLA-DPB1 ANTIBODIES IN RENAL TRANSPLANTATION
JC Kelleher, D Keegan, K Khalib, S Jennings, D O'Neill, M Keogan (Dublin)

O35 ANTIBODIES AGAINST DONOR HLA OR MICA ARE ASSOCIATED WITH REJECTION OF HEART ALLOGRAFTS
Y Zou, Z Qin, H Lee, LS Hynan, B Lavingia, P Stastny (Dallas, TX)

O36 THREE YEARS FOLLOW-UP OF KIDNEY ALLOGRAFT RECIPEINTS WITH CONCURRENT DONOR CELLS INFUSION
G Solgi, J Mytilineos, V Gadi, B Paul, G Pourmand, M Ranjbar, B Nikbin, A Amirzargar (Hamadan)

O37 PECAM-1-DEPENDENT REGULATION OF HEME OXYGENASE-1 VIA THE ANTIOXIDANT NRF2 PATHWAY IN THE ENDOTHELIUM
H Saragih, E Zilian, Y Jaimes, C Figueiredo, B Eiz-Vesper, R Blasczyk, J Larmann, G Theilmeier, S Santoso, S Immenschuh (Hannover)

O38 CLASSICAL HLA TYPE IMPUTATION FOR MULTI-POPULATION STUDIES
AT Dilthey, S Leslie, J Shen, L Moutsianas, M Nelson, G McVean (Oxford)

O39 DECIPHERING HLA AND KIR ALLELES FROM WHOLE-GENOME DATA
PJ Norman, A Moudgil, BM Henn, JM Kidd, JD Wall, C Bustamente, P Parham (Stanford)

O40 QUANTIFYING THE CONTRIBUTION OF POLYMORPHIC RESIDUES TO PEPTIDE BINDING OF HLA MOLECULES.
HW van Deutekom, C Kesmir (Utrecht)

Oral IV - Non HLA Polymorphism/Population Genetics/Anthropology - Hall 1c : Saturday, 2nd June, 11:00-13:00
O41 ORANGUTAN KIR HAPLOTYPE VARIATION
LA Guethlein, L Abi-Rached, PJ Norman, F Babrzadeh, NG de Groot, RE Bontrop, P Parham (Stanford, CA)

O42 ONE EUROPEAN REFERENCE FOR STR DIVERSITY: POPULATION GENETICS OF 15 IDENTIFILER STR MARKERS IN 9 EUROPEAN COUNTRIES.
M Groeneweg, CE Voorter, MG Tilanus (Maastricht)

O43 DEVELOPMENT OF SUPER HIGH RESOLUTION SINGLE MOLECULE - SEQUENCE BASED TYPING (SS-SBT) METHOD AT THE 8-DIGIT LEVEL WITHOUT AMBIGUITY IN HLA CLASS II GENES
Y Ozaki, S Suzuki, E Kikkawa, A Shigenari, A Oka, S Mitsunaga, T Shiina, H Inoko (Isehara, Kanagawa)

O44 “IMMUNOGENETICS OF AGEING” - CONTRIBUTION OF INNATE IMMUNITY GENES TO UNDERSTANDING THE IMMUNE DYSFUNCTION IN ELDERLY
E Naumova, MI Ivanova, G Pawelec, D Middleton, F Qguz, I Constantinescu, A Georgieva, C Caruso (Sofia)

O45 KIR SIGNATURE ON SPECIFIC HLA-A,B HAPLOTYPES IN CAUCASIANS
C Capittini, M Guarene, A Pasi, C Badulli, E Kurici, I Sbarsi, M Monti, F Garlaschelli, A Cremaschi, C Pizzochero, C Tinelli, L Salvaneschi, M Martinetti (Pavia)

O46 SOLUBLE MICA-NKG2D INTERACTION PROMOTES IFN-GAMMA PRODUCTION BY CD56BRIGHT NK CELLS WITHOUT INDUCING CYTOTOXICITY
W Boukouaci, M Busson, C Fortier, R Al-Daccak, N Dulphy, R Peffault de Latour, G Socié, A Toubert, D Charron, R Krishnamoorthy, R Tamouza (Paris)

O47 COPY NUMBER VARIATION LEADS TO CONSIDERABLE DIVERSITY FOR KIR B, BUT NOT A, HAPLOTYPES.
W Jiang, C Johnson, J Jayaraman, J Trowsdale, JA Traherne (Cambridge)

O48 IL28B, KIR GENES AND HLA LIGANDS IN CHRONIC HEPATITIS C VIRUS INFECTION BEFORE AND AFTER LIVER TRANSPLANTATION
C Bandelier, P Bochuz, C Gbame, S Rodel, J Dufour, B Mulhaupt, G Mentha, F Negro, E Giostra, J Villard (Geneva)

O49 KIR3DL1HIGH NK CELL FREQUENCY IS NOT INFLUENCED BY AUTOLOGOUS BW4 LIGAND BUT IS DETERMINED BY A STOCHASTIC AND ALLELIC KIR3DL1 EXCLUSION AND CO-EXPRESSION OF BOTH KIR3DL1 ALLELES.
K Gagne, C Willem, N Legrand, Z Djaoud, G David, A Cesbron Gautier, T Schneider, C Retiere (Nantes)

O50 EVOLUTONAL MEDICINE IS AN APPROAH TO IDENTIFY THE HUMAN GENOME DIVERSITY ASSOCIATED WITH HIV/AIDS
A Kimura, H Ohtani, T Naruse, T Nakajima, K Mori, H Akari, T Matano, G Sharma, G Kaur, NK Mehra (Tokyo)

Oral V - MHC-peptides -Epitopes and Prediction/Antigen Presentation and Immunological Tolerance - Hall 1b : Sunday, 3rd June, 11:00-13:00
O51 THE STRUCTURAL CONSTRAINT OF THE PEPTIDE PATTERN FOR B*44:06 CAN BE ATTRIBUTED TO RESIDUE 63 IN THE HEAVY CHAIN
T Huyton, C Bade-Doeding, S Badrinath, H Schumacher, R Blasczyk, C Bade-Doeding (Hannover)

O52 IMMUNE INDUCED EVOLUTIONARY SELECTION FOCUSED ON A SINGLE READING FRAME IN OVERLAPPING HBV PROTEINS.
Y Louzoun, Y Maman (Ramat Gan)

O53 VIRUSES AND BACTERIA SELECTIVELY MUTATE THEIR CD8+ T CELL EPITOPES - A LARGE SCALE IMMUNOMIC ANALYSIS.
Y Louzoun, Y Maman (Ramat Gan)

O54 SEPARATE DEVELOPMENTAL PROGRAMS FOR HLA-A AND -B EXPRESSION DURING DIFFERENTIATION FROM EMBRYONIC STEM CELLS TO LYMPHOCYTES, ADIPOCYTES AND OSTEOBLASTS
HJ Sabir, J Nehlin, A Isa, T Barington (Odense C)

O55 ORAL GENE THERAPY VIA MAJOR-HISTOCOMPATIBILITY-COMPLEX (MHC) ENCODING CHITOSAN-DNA NANOPARTICLES REDUCES TRANSPLANTARTHERIOSCLEROSIS IN A MURINE AORTIC ALLOGRAFT MODEL
K Goldmann, J Hofmann, S Eckl, EM Stephan, BM Spriewald (Erlangen-Nürnberg)

O56 COMPLEX AND DIVERGENT PATTERNS OF AMINO ACID RESIDUES ARE INVOLVED IN T CELL ALLOREACTIVITY TO HLA-DP9
P Crivello, L Zito, F Sizzano, A Mulder, E Zino, L Naldini, K Fleischhauer (Milan)

O57 HIGH THROUGHPUT, HIGH FIDELITY HLA GENOTYPING WITH ULTRA DEEP SEQUENCING
S Krishnakumar, C Wang, J Wilhelmy, F Babrzadeh, L Su, D Levinson, M Fernandez-Vina, R Davis, M Davis, MN Mindrinos (Palo Alto)

O58 PROPERTIES OF MHC CLASS I PRESENTED PEPTIDES THAT ENHANCE IMMUNOGENICITY
JJ Calis, C Kesmir, B Peters (Utrecht)

O59 EVOLUTION OF THE ALTERNATIVE CLASSIFICATION OF HLA-DRB1 ALLELES IN RHEUMATOID ARTHRITIS: IMPACT ON THE PRODUCTION OF THE ANTI-CITRULLINATED PROTEIN ANTIBODIES AND STRUCTURAL PROGRESSION, DATA FROM THE ESPOIR COHORT
A Ruyssen-Witrand, PA Gouraud, D Nigon, C Lukas, C Miceli-Richard, B Jamard, A Cantagrel, A Cambon-Thomsen, P Dieudé, A Constantin (Toulouse)

O60 STIMULATION OF EBV AND CMV SPECIFIC CYTOLYTIC EFFECTOR FUNCTION USING ALLOGENEIC CELL IMMUNOTHERAPY
LJ D'Orsogna, H van den Heuvel, DL Roelen, E van der Meer-Prins, II Doxiadis, F Claas (Leiden)

Oral VI - Immunogenetics/Miscellaneous - Hall 1c : Sunday, 3rd June, 11:00-13:00
O61 DETERMINING GENE COPY NUMBER VARIATION IN THE KILLER IMMUNOGLOBULIN-LIKE RECEPTOR FAMILY USING MULTIPLEX LIGATION-DEPENDENT PROBE AMPLIFICATION
S Vendelbosch, M de Boer, R Gouw, CK Ho, WT Swelsen, TK van den Berg, TW Kuijpers (Amsterdam)

O62 UTILITY OF NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN (NGAL) IN EARLY DETECTION OF ACUTE RENAL INJURY
PS Dhurandhar, AR Halankar, HB Chafle (Raja Rammohan Roy Road, Prarthana Samaj, Mumbai)

O63 HLA GENOTYPING ON THE PERSONAL GENOME MACHINE (ION TORRENT) MAY BE THE NEXT GENERATION IN THE HLA LABORATORY.
D De Santis, N Kresoje, I Vukovic, A Mojas, CS Witt, D Sayer, D Goodridge, P Martinez, R Allcock (Perth)

O64 POLYMORPHISMS IN TWO OF ULBP2/RAET1H GENES IN THE OLD WORLD MONKEY
T Naruse, K Mori, H Akari, T Matano, A Kimura (Tokyo)

O65 SEMAPHORIN RECEPTORS FACILITATE STIMULATION OF ALLOREACTIVE T HELPER CELLS
L Schlahsa, B Anja, S Vahlsing, B Eiz-Vesper, S Immenschuh, R Blasczyk, C Figueiredo (Hannover)

O66 EVOLUTION OF HLA-DRB GENES
GG Doxiadis, I Hoof, N de Groot, RE Bontrop (Rijswijk)

O67 NKG2A AND NKG2C DEPENDENT NK CELL ACTIVATION IS REGULATED BY HLA-E EXPRESSION LEVELS AND THE PEPTIDE PRESENTED BY HLA-E.
N Lauterbach, L Wieten, L van Zon, CE Voorter, MG Tilanus (Maastricht)

O68 INVESTIGATION OF THE ROLE OF KILLER CELL IMMUNOGLOBULIN-LIKE RECEPTOR POLYMORPHISMS IN NATURAL KILLER CELL FUNCTION
TM Myint, HF North, S Cassidy, CJ Brown, SI Khakoo (London)

O69 ORIGIN SPECIFIC ADAPTATION OF KIR IN NON-HUMAN PRIMATES AS EVIDENCED BY NEXT-GENERATION SEQUENCING
JH Blokhuis, MK van der Wiel, GG Doxiadis, RE Bontrop (Rijswijk)

O70 DEFINING EARLY HUMAN NK CELL DEVELOPMENTAL STAGES IN PRIMARY AND SECONDARY LYMPHOID TISSUES
DN Eissens, A van der Meer, J Spanholtz, B van Cranenbroek, H Dolstra, J Kwekkeboom, F Preijers, I Joosten (Nijmegen)